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Sterilization is a process that eliminates, removes, kills, or deactivates all forms of life and other biological agents (such as fungibacteriaviruses, spore forms,) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media.


Disinfection is a process of killing of bacteria or micro-organisms on non-living /in animate objects or surfaces which does not include killing bacterial spores.


Asepsis is the state of being free from disease-causing micro-organisms (such as pathogenic bacteria, viruses, pathogenic fungi, and parasites).

Asepsis is maintained in hospitals, surgical rooms , dental clinics , etc to keep the environment germ free.